Genomics & Bioinformatics Core Facility

Covaris Fragmentation

Illumina recommends the Covaris focused-ultrasonicator system to fragment DNA in preparation of DNA sequencing libraries. Ultrasonication is favored over other fragmentation methods because DNA molecules are randomly broken with minimal bias. Ultrasonication avoids the limitation observed with Nebulization and enzymatic methods. The Genomics Core facility provides DNA fragmentation services using the Covaris S220 Ultrasonicator System. Nucleic acid fragmentation is applicable to many applications including chromatin shearing, tissue homogenization, cell lysis, compound dissolution, and particle micronization. For custom services, contact us.

Fragmentation Parameters

DNA fragmentation with the S220 produces consistent, reproducible results. To achieve the desired average size distribution, refer to the table to choose the appropriate Adaptive Focused Acoustics (AFA) tubes. Selecting the appropriate fragmentation parameters is sample dependent. See the Covaris DNA Shearing Guide for more information.

Covaris AFA Tubes
Range of Fragment Size Distribution
Recommended Sample Volume
microTUBE
150bp – 1,500bp
130ul
microTUBE
150bp – 1,500bp
50ul
miniTUBE
2- 5kb
200ul
g-TUBE
6 – 20kb
50ul

Sample Preparation

For each sample to be processed, provide your sample in labeled 1.5ml tubes. Include Your Name, Date (MM/DD/YYYY), and Sample Name. Submit the samples and submission form to our facility. After service completion, the fragmented sample is returned in a sterile 1.7mL microcentrifuge tube and documentation of parameters is provided. 

Sample Submission Form

Contact us to schedule delivery of your samples to our Genomics Core Facility.

Genomics Core Facility
Galvin Life Science Center Room 019
Phone: (574) 631-1902