Genomics & Bioinformatics Core Facility

DNA Sanger Sequencing Ready to Run

Our Ready-to-Run Service option includes samples ready to sequence on the Applied Biosystems 96-capillary 3730xl DNA Analyzer. The user completes the DNA Sanger reaction preparation and submits the samples according to our Sample Preparation Guidelines. Please note sample plates are discarded after data is delivered.

Looking for resources to help you troubleshoot your DNA sequencing reactions? Please see our Troubleshooting Resources page. 


Submitting Your Order

Genomics Core Facility manages all DNA Sanger Sequencing Service using two interfaces: CORES Ordering System and Sample Information Form.
For 2- to 3-day business day service, enter your order into the CORES Ordering System and send your Ready-to-Run Sample Information Form to genomics@nd.edu by 12:00PM, and samples must be delivered to our faclity by 2:00PM. 

CORES Order Entry

NEED TO SETUP A NEW RESOURCE USER IN CORES: Resource guides are available from the Facilities and Resouces page of Notre Dame Research.

  1. Login to CORES.
  2. From the Order Entry Panel, enter into the field your Last Name or FOP. Click Search button.
  3. Select the appropriate CORE Facility: Genomics Core Facility - Pfrender, Michael
  4. Select the appropriate FOP from the list.
  5. Complete the Order Entry –Item Information:
    1. Select the Category from the drop-down menu
    2. Select the Description from the drop-down menu
    3. Enter the quantity.
  6. Click Add Item button to entry additional items to the same order, and then repeat step 5.
  7. REQUIRED: Purchaser’s Information must be populated with your Last Name, First Name, email address, and phone number.
    1. Choose your own name from the Purchaser's drop down menu. If your name is not populated in the drop down, replace the default Last Name and First Name fields with your information. When the order is submitted, data will be delivered to the Purchaser Listed. Failure to complete the Purchaser’s Information will delay sample processing.
  8. Review your Order Entry. Be sure all information is accurate and the Purchaser’s Information correct.
  9. Click Submit Order Request.

Ready-to-Run Sample Information Form

  1. Download Ready-to-Run Sample Information Form for DNA Sanger Sequencing.
  2. Send your completed form to genomics@nd.edu by 12:00PM for 2- to 3-day business day service.

Sample Delivery

To avoid sample-processing delays, ensure your tubes/plates are clearly labeled with your initials, date, and sample number. Deliver your samples to our Genomics Core Facility by placing them in our designated DNA Sanger Sequencing freezer. Please note sample tubes/plates and primer tubes are discarded after data is delivered.

Deliver your samples by no later than 2:00PM (EST-D) to our facility:

Genomics Core Facility
Galvin Life Science Center Room 019
Phone: (574) 631-1902

Sample Preparation Guidelines

  1. For ready-to-run samples, submit your DNA Sanger Sequencing products in a labeled 3730xl-compatible 96-well plate suspended in the appropriate volume of AB Hi-Di Formamide. For a list of 3730xl-compatible 96-well plates, please see the AB3730xl Compatible Plates section.
  2. Submit your samples and order form according to the Order Submission Guidelines and Sample Submission Guidelines.

AB3730 Compatible 96-well Half-Skirted Plates

Distributor

Dist. Catalog

Description

Dot Scientific, Inc.
351-PCR
PCR PLT 96W 0.2ML HS NAT PRINTED AXIS 10/PK
ThermoFisher Scientific
AB1400
Thermo Scientific 96-Well Semi-Skirted Plates, Flat Deck

Getting Your Results 

Sanger sequencing data files are posted to Google Drive. Need permission to access your files? Reach out to us by completing our Sanger Sequencing Request Access Form
 
If you have questions about your sanger data files, please contact Genomics Core Facility at 574-631-1902 or genomics.nd.edu.
Working in Google Drive
For additional information, Google Drive quick-reference guides and resources are available to help you get started. 
 
Want to map your computer directly to your Sanger Sequencing data folder? You will need to add the shared folder to your "My Drive" first and then install Google File Stream on your computer by following these instructions
 
If you have any questions about Google Drive, please contact your departmental IT support staff, or OIT Help Desk at 574-631-8111, oithelp@nd.edu or chat online at: help.nd.edu.
 
Viewing Your Results

Sanger Sequencing Software

Applied Biosystems Sequence Scanner Software v2.0 (Download)
Operating System: Windows 7
Description: View, edit, print and export data generated using AB37030XL and other Applied Biosystesms Genetic Analyzer instruments. 

QIAGEN CLC Sequence Viewer (Download)
Operating System: Windows Vista, Windows 7, Windows 8, Windows 10, Windows Server 2008, or Windows Server 2012; Mac OS X 10.7 or later
Description: View, edit, and manipulate data generated using AB37030XL and other Applied Biosystems Genetic Analyzer instruments. For a complete list of features, click here

Sequencer 5.1 (Download)
(Notre Dame Faculty / Staff / Students only)
Operating System: Windows; Mac OS X
Description: View, edit, print and export data generated using AB37030XL and other Applied Biosystesms Genetic Analyzer instruments.
Required Utility Software:  To run Sequencher, KeyAccess client must be installed. Downolad here.
Remote Access Uility Software: To remotely access Sequencher, Cisco Clean Access VPN connection is required when off-campus. Download here.
 

Fragment Analysis Software

Applied Biosystems Peak Scanner Software v2.0 (Download)
Operating System: Windows 7
Description: DNA sizing software to perform fragment analysis, separate a mixture of DNA fragments by size and provide a profile of the seperation. View, edit, print and export data generated using AB37030XL and other Applied Biosystesms Genetic Analyzer instruments. 

Applied Biosystems Sizging Analysis Module, Peak Scanner Software (Go to Cloud)
Operating System: Free access on Thermo Fisher Cloud
Description: DNA sizing software to perform fragment analysis, separate a mixture of DNA fragments by size and provide a profile of the seperation. View, edit, print and export data generated using AB37030XL and other Applied Biosystesms Genetic Analyzer instruments. 

Resources

Ready-to-Run Sample Information Form