Genomics & Bioinformatics Core Facility

Speciality Workflows

Introduction

The GBCF offers a variety of standard and custom workflows for NGS library creation and Illumina sequencing. Please check out our Services page for a list of services offered. We also specialize in several workflows. To learn more about our services please contact us.

Speciality Service Options

Double Digest Restriction-site Associated DNA Preparation (ddRAD-tag)

Restriction-site associated DNA (RAD) Library Preparation is a type of Reduced Representation Library (RRL) that analyzes DNA markers, or tags, near restriction digest sites. RAD-tag sequencing has been utilized in studies of Quantitative Trait Loci (QTL) mapping, genotype by sequencing, Single-Nucleotide Polymorphism (SNP) analysis, and population and ecological genetics. Our RAD-tag library preparation is compatible with Illumina sequencing. Resources are available for experimental design, library preparation, and data analysis. 

Environmental DNA Sequencing

Using genetic material directly obtained from environmental samples (soil, water, etc) is an efficient and non-invasive way to sample biodiversity of an ecosystem. This technique for library generation and sequencing has been utilized in biodiversity monitoring and conservation and detecting rare and invasive species. Our technicians have experience working with and sequencing these challenging and low input samples.

Amplicon Sequencing (Metagenetics)

16S ribosomal RNA (rRNA) sequencing is a common amplicon sequencing method used to identify and compare bacterial present with a given sample. It is a well-established method for studying phylogeny and taxonomy of samples from complex microbiomes or environments that are difficult to study. PCR-based approaches enable analysis of the entire mcrobial community within a sample with the ability to combine many samples in a sequencing run. We offer a streamlined workflow for 16S rRNA coving the V3, V4 hypervariable regions for microbiome sequencing as well as support for other markers such as 12S, 18S, ITS, and COI for metagenetics and eDNA applications.