“An introduction to home-brew library prep methods for Illumina sequencing and critical factors to ensure success: ChIP-Seq, ATAC-Seq, Hi-C, and CRISPR screening”

In this webinar, we will discuss popular applications that utilize home-brew library preps with Illumina Sequencing as a downstream readout. This will include an overview of epigenetic methods designed to look at regions of open chromatin (ATAC-Seq), binding sites of DNA-associated proteins (ChIP-Seq/Cut and Run), and detection of long-range DNA interactions (Hi-C). Additionally, we will look at how Illumina Sequencing can be used as an effective strategy in genome wide CRISPR screens for quantification of sgRNAs as well as methods that allow the detection of transcriptional changes observed in the enriched or depletion knockout populations.

Speaker name and title: Lauren Robinson, Sequencing Specialist

Speaker institution: Illumina, Inc.

Hosted by:

• Indiana University Center for Genomics & Bioinformatics, Matthew Hahn, PhD, Director
• Indiana University School of Medicine Center for Medical Genomics, Yunlong Liu, PhD, Director
• Notre Dame Genomics & Bioinformatics Core Facility, Michael Pfender, PhD, Director
• Purdue University Bindley Core for Genomics, Phillip SanMiguel, PhD, Director

Please register to receive the ZOOM meeting link: redcap.link/CTSI_Technology_Seminar