Illumina Sequencing

The GBCF is dedicated to providing high-quality service and offering flexible options that allow personalized service to users. The Core offers Illumina Sequencing using the Illumina MiSeq and NextSeq 500 sequencing platforms. Additionally, the GBCF is equipped to handle most sample types and workflows offering a variety of library preparation solutions, sequencing, and data analysis solutions for characterizing the genome, epigenome, and transcriptome. Our highly trained NGS scientists have direct experience and knowledge operating complex technical instrumentation and balancing the throughput needs of our research community. 

Service Options

In addition to on-site Illumina MiSeq and NextSeq 500 Sequencing Services, the GBCF is able to offer alternative sequencing solutions through collaborative pricing with other institutions.

MiSeq Sequencing

The MiSeq desktop sequencer achieves the longest read length of any Illumina instrument. Its single lane and kit flexibility make it ideal for reduced representation studies and focused applications such as microbial profiling, targeted gene sequencing, small genome, and amplicon sequencing.

NextSeq Sequencing

The NextSeq 550 is a high-throughput benchtop sequencer that offers fast turnaround time and flexibility with run configurations. There are two types of flow cell options (mid and high output) available with a range of outputs to accommodate a wide variety of NGS projects. NextSeq 550 is the ideal platform for small to medium sequencing projects that range in output requirements.

View MiSeq and NextSeq 500 comparison tables.

Service Rates

View internal and external service rates

Submitting Your Order

Consultation is required for Illumina sequencing services. Please contact Melissa Stephens before preparing samples to discuss the project goals.

Sequencing Only (User Prepared) Illumina Library Submissions

  1. For user prepared submissions, 20 ul of prepared library at a concentration of 10nm is required. The library should be placed in 1.5mL tubes. Label each tube with your initials, date (MM/DD/YYYY), and Library ID.
  2. If the libraries are multiplexed, please include the list of the index barcodes used.
  3. Upon receipt, GBCF staff will confirm the library concentration using a combination of Qubit, Bioanalyzer analysis, and Kapa qPCR Library Quantitation.

Sample Storage

After sequencing is complete, the GBCF will store completed libraries for a period of no longer than six months before discarding. Please make arrangements with the GBCF to have any remaining material, including leftover nucleic acids, returned to you for longer term storage.