10X Chromium Controller
The ability to examine genomic and transcriptomic patterns at the level of single cells is rapidly becoming an essential tool for cell biology, cancer, immunology, and neuroscience research.
The 10X Chromium Controller is a massive partitioning and barcoding system with a reagent delivery method that facilitates thousands of micro-reactions in parallel. This enables the discovery of gene expression dynamics and molecular profiling of individual cell types, elucidation of gene regulatory mechanisms, study of disease pathogens, and characterization of genomic structure.
A variety of library preparation options are available for a wide range of study goals. Researchers are encouraged to contact the GBCF with requests for other services that are not listed below:
- Single Cell Gene Expression
- Gene Expression CRISPR Screening
- Single Cell Immune Profiling
- Single Cell CNV
- Single Cell ATAC
- Gene and Cell Surface Proteins
- Spatial Transcriptomics
- Long Range (Linked-Read) Genome sequencing
New Policies and Procedures
Effective January 01, 2021
External Users: Contact Melissa Stephens. We are accepting a limited number of requests until further notice.
Internal Users: Access Galvin Life Science Center via the main entrance during regular business days and hours.
iLab transition complete as of January 06, 2021. Go to InsideND and search for iLab Core Facility Management. GBCF iLab video tutorials and user guide available on Resource tab.
Communicate with facility staff by email, phone, or Zoom appointment.
Samples delivered contactless. Access to facility space is restricted to facility staff. On designated days and reserved times, there will be a cooler outside the facility door to receive samples. Do not leave samples if the cooler is absent; we are not responsible for damage or loss.
Submitting Your Order
Consultation is required for this service. Before initiating your experiment, contact Melissa Stephens to discuss project specifics, receive a quote, and coordinate scheduling. Once your request is confirmed and scheduled, follow the instructions for library preparation below.
Single-Cell Library Preparation Submissions
Confirmed and Scheduled Requests
Core Facility will perform its own evaluation of the samples to confirm (1) concentration and (2) purity using an automated cell counter.
Project Information: Download and complete all fields to request guide discussion and development of your request. (Coming Soon).
Sample Information and QC: Download and complete all fields to submit your samples for evaluation. (Coming Soon.)
Sample requirements are specific to the project and will be outlined during the discussion and development phase prior to confirming and scheduling.
iLab Core Facility Management: Reserve a sample drop-off day/time. iLab sends us a notification about scheduled reservations. Do not send us a separate email.
Go to InsideND, search for iLab Core Facility Management, and sign in using University of Notre Dame credentials.
Schedule a reservation 24 hours in advance by selecting a day and time from the Sample Processing Calendar and attach your completed Excel Submission Form. Within the service request, including the email of other lab members who should be consulted or notified with results. Within the reservation, include the email of other lab members who should be consulted or notified with results.
REQUIRED: If you can not make your reservation, cancel it as soon as possible and select a new time slot.
Recommendation: Coordinate within your lab to reduce multiple deliveries to a single drop-off reservation whenever possible.
Deposit the sample inside the cooler outside of the facility door on your reserved day and time. REQUIRED: Enclose tube(s) inside a small biohazard bag.
On the day of processing, you must be available for a phone call, email, or Zoom meeting to discuss results.
Turn around time is provided during the discussion and development phase.