DNA Sanger Sequencing Ready to Run

With the DNA Sanger Sequencing Ready to Run, the user submits the sequencing reaction (or Fragment Analysis) ready to load on the Applied Biosystems 96-capillary 3730xl DNA Analyzer.

Service Rates

View internal and external service rates

New Policies and Procedures

Effective January 01, 2021


External Users: Contact Melissa Stephens. We are accepting a limited number of requests until further notice.

Internal Users: Follow all University Re-opening procedures. Access Galvin Life Science Center via the main entrance during regular business days and hours. 

  1. iLab transition complete as of January 06, 2021. Go to InsideND and search for iLab Core Facility Management. Any order submitted to CORES after January 01, 2021 will not be fulfilled.

  2. Communicate with facility staff by email, phone, or Zoom appointment. In-person meetings are restricted until further notice.

  3. Samples delivered contactless. Access to facility space is restricted to facility staff.  On designated days and reserved times, there will be a cooler outside the facility door to receive samples. Do not leave samples if the cooler is absent; we are not responsible for damage or loss.

Submitting Your Order

  1. Guidelines for Submission: Review and prepare samples accordingly. (Coming Soon.)

  2. Excel Submission Form: Download and complete all fields. REQUIRED: Attach it to iLab service request. 

  3. iLab Core Facility Management: Request service and reserve a sample drop-off day/time. iLab sends us a notification about service requests and scheduled reservations. Do not send us a separate email.

    • Go to InsideND, search for iLab Core Facility Management, and sign in using University of Notre Dame credentials.

    • Request service and attach your completed Excel Submission Form. Within the service request, include the email of other lab members who should be consulted or notified with results. 

    • Schedule a reservation 2 hours in advance by selecting a day and time from the Sample Processing Calendar.

      • REQUIRED: If you can not make your reservation, cancel it as soon as possible and select a new time slot.

      • Recommendation: Coordinate within your lab to reduce multiple deliveries to a single drop-off reservation whenever possible.

  4. Deposit the sample inside the cooler outside of the facility door on your reserved day and time.

AB3730 Compatible 96-well Half-Skirted Plates

Distributor

Dist. Catalog

Description

Dot Scientific, Inc.

351-PCR

PCR PLT 96W 0.2ML HS NAT PRINTED AXIS 10/PK

ThermoFisher Scientific

AB1400

Thermo Scientific 96-Well Semi-Skirted Plates, Flat Deck

Your Results

When your service request is completed, an email notification is sent to the person who created it.
Results are posted to Google Drive. If permission is needed to access results, please indicate within the Excel Submission Form.

To map your computer directly to the Sanger Sequencing data folder, you will need to add the shared folder to "My Drive" first and then install Google File Stream on your computer by following these instructions. For additional information, Google Drive quick-reference guides and resources are available. If you have any questions about Google Drive, please contact the OIT Help Desk.

Software

There is a variety of sequencing and fragment analysis software available to view results. View the list of available software.

Sequencing Failed Reactions / Redo Policy

Rerun service is not provided with Ready-to-Run orders, with rare exceptions.

Troubleshooting Resources

The GBCF staff has compiled links that are helpful for troubleshooting DNA sequencing reactions. Please contact the GBCF if you are unable to find what you're looking for. View DNA Sequencing by Capillary Electrophoresis Troubleshooting Guide.

Additional Resources