Covaris Fragmentation

The GBCF provides DNA fragmentation services using the Covaris S220 Ultrasonicator System. Nucleic acid fragmentation is applicable to many applications including DNA sequencing library preparation, chromatin shearing, tissue homogenization, cell lysis, compound dissolution, and particle micronization. Ultrasonication is favored over other fragmentation methods because DNA molecules are randomly broken with minimal bias.

Service Rates

View internal and external service rates 

NEW Policies and Procedures Regarding Sample Drop Off Services

  • Each lab will be requested to schedule a specific drop off time to deposit samples outside of the facility for processing (see 'submitting your order' below). A cooler will be placed outside the door for this purpose. This procedure is an effort to reduce traffic inside and outside the lab.

  • Please coordinate multiple submissions with your lab members whenever possible to limit the traffic outside of the lab.

  • External orders will not be accepted at this time. 

  • Please maintain at least a 6 foot distance from others whenever possible. A mask must be worn at all times while in the building.

Submitting Your Order

  1. Download the Covaris Submission Form and e-mail the completed copy to genomics@nd.edu. In your e-mail, please include the date and approximate time you will be delivering your samples to the GBCF.

  2. Review the Sample Preparation Guidelines for preparing tubes.

  3. Please choose a time to drop off your samples using the GBCF Daily Sample Drop Off Schedule each week.

  4. Deposit the samples outside of the GBCF and inside the cooler using the provided tube rack on the day and time that was selected.

If you are unable to meet your drop off day and time, please reschedule with your new anticipated drop off time as soon as possible.

 

 

Your Results

After service completion, the fragmented sample is returned in a sterile 1.7mL microcentrifuge tube and documentation of parameters is provided.