DNA Sanger Sequencing Full Service
DNA Sanger Sequencing (Capillary Sequencing) Full Service is used by researchers who wish to submit DNA and primer for full service, which includes DNA Sanger reaction preparation, reaction clean-up, and sequencing.
Service Rates
View internal and external service rates.
New Policies and Procedures
Effective January 01, 2021
External Users: Contact Melissa Stephens. We are accepting a limited number of requests until further notice.
Internal Users: Access Galvin Life Science Center via the main entrance during regular business days and hours.
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iLab transition complete as of January 06, 2021. Go to InsideND and search for iLab Core Facility Management. GBCF iLab video tutorials and user guide available on Resource tab.
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Communicate with facility staff by email, phone, or Zoom appointment.
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Samples delivered contactless. Access to facility space is restricted to facility staff. On designated days and reserved times, there will be a cooler outside the facility door to receive samples. Do not leave samples if the cooler is absent; we are not responsible for damage or loss.
Submitting Your Order
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Guidelines for Submission: Review and prepare samples accordingly.
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Excel Submission Form: Download and complete all fields. REQUIRED: Attach it to iLab service request.
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iLab Core Facility Management: Request service and reserve a sample drop-off day/time. iLab sends us a notification about service requests and scheduled reservations. Do not send us a separate email.
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Go to InsideND, search for iLab Core Facility Management, and sign in using University of Notre Dame credentials.
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Request service and attach your completed Excel Submission Form. Within the service request, including the email of other lab members who should be consulted or notified with results.
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Schedule a reservation 1 hour in advance by selecting a day and time from the Sample Processing Calendar.
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REQUIRED: If you can not make your reservation, cancel it as soon as possible and select a new time slot.
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Recommendation: Coordinate within your lab to reduce multiple deliveries to a single drop-off reservation whenever possible.
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Deposit the sample inside the cooler outside of the facility door on your reserved day and time.
Your Results
When your service request is completed, an email notification is sent to the person who created it.
Results are posted to Google Drive. If permission is needed to access results, please indicate within the Excel Submission Form.
To map your computer directly to the Sanger Sequencing data folder, you will need to add the shared folder to "My Drive" first and then install Google File Stream on your computer by following these instructions. For additional information, Google Drive quick-reference guides and resources are available. If you have any questions about Google Drive, please contact the OIT Help Desk.
Software
There is a variety of sequencing and fragment analysis software available to view results. View the list of available software.
Sequencing Failed Reactions / Redo Policy
Read lengths of up to 750bp at QV >20 can be expected. If the full-service sequencing reaction produces ambiguous results, a researcher may request for the GBCF to rerun the samples. These will be reviewed on a case-by-case basis. When repeating the reaction, the sample tubes and primers used must be the same used in the initial reaction. If the reaction produces a better result, there is no additional cost. However, if the reaction fails to produce a different result, the provided FOAPAL will be charged for the second attempt. *For plate orders, rerun service is not provided, with rare exceptions
Troubleshooting Resources
The GBCF staff has compiled links that are helpful for troubleshooting DNA sequencing reactions. Please contact the GBCF if you are unable to find what you're looking for or view the DNA Sequencing by Capillary Electrophoresis Troubleshooting Guide.