DNA Sanger Sequencing Full Service

DNA Sanger Sequencing (Capillary Sequencing) Full Service is used by researchers who wish to submit DNA and primer for full service, which includes DNA Sanger reaction preparation, reaction clean-up and sequencing.

Service Rates

View internal and external service rates

NEW Policies and Procedures Regarding Sample Drop Off Services

  • Full Service Sanger sequencing will be offered weekly on Tuesdays and Fridays, starting June 12. 

  • Order forms must be submitted and samples delivered to the core by no later than 2:00pm to be eligible for processing on the next available sequencing day.

  • Each lab will be requested to schedule a specific drop off time to deposit samples outside of the facility for processing. A cooler will be placed outside the door for this purpose. This procedure is an effort to reduce traffic inside and outside the lab.

  • Please coordinate multiple submissions with your lab members whenever possible to limit the traffic outside of the lab.

  • External orders will not be accepted at this time. 

  • Please maintain at least a 6 foot distance from others whenever possible. A mask must be worn at all times while in the building.

Submitting Your Order

  1. Enter the order into the CORES Ordering System

  2. Review the Sample Preparation Guidelines for preparing tubes.
  3. Download the Full Service Sample Information Form and e-mail the completed copy to genomics@nd.edu.
  4. Please choose a time to drop off your samples using the GBCF Daily Sample Drop Off Schedule each week.
  5. Deposit the samples outside of the GBCF and inside the cooler using the provided tube rack on the day and time that was selected.
If you are unable to meet your drop off day and time, please reschedule with your new anticipated drop off time as soon as possible.

 

Your Results

An e-mail is sent when data is available. Sanger sequencing data files are posted to Google Drive. If permission is needed to access the files, please contact the GBCF staff by completing the Sanger Sequencing Request Access Form

To map your computer directly to the Sanger Sequencing data folder, you will need to add the shared folder to "My Drive" first and then install Google File Stream on your computer by following these instructions. For additional information, Google Drive quick-reference guides and resources are available. If you have any questions about Google Drive, please contact the OIT Help Desk.

Software

There are a variety of sequencing and fragment analysis software available to view results. View list of available software.

Sequencing Failed Reactions / Redo Policy

Read lengths of up to 750bp at QV >20 can be expected. If the full service sequencing reaction produces ambiguous results, a researcher may request for the GBCF to rerun the samples. These will be reviewed on a case-by-case basis. When repeating the reaction, the sample tubes and primers used must be the same used in the initial reaction. If the reaction produces a better result, there is no additional cost. However, if the reaction fails to produce a different result, the provided FOAPAL will be charged for the second attempt. *For plate orders, rerun service is not provided, with rare exceptions

Troubleshooting Resources

The GBCF staff has compiled links that are helpful for troubleshooting DNA sequencing reactions. Please contact the GBCF if you are unable to find what you're looking for or view the DNA Sequencing by Capillary Electrophoresis Troubleshooting Guide.

Additional Resources