Illumina Library Preparation

The GBCF is dedicated to providing high-quality service and offering flexible options that allow personalized service to users. The Facility offers NGS Library Preparation Services compatible with Illumina Sequencing Platforms using both commercial kits and custom workflows. 

Standard & Specialty Service Options

A variety of library preparation options are available for a wide range of sample types. Researchers are encouraged to contact the GBCF with requests for other services not listed below, as the Facility may provide additional custom services.

Genomics (de novo assembly, resequencing)

  • Whole Genome DNA
  • ddRAD-tag libraries
  • Mate-Pair or Synthetic Long Reads
  • Target Enrichment

 Transcriptomics (Total RNA, mRNA, FFPE)

  • Classic strand-specific RNA (mRNA or rRNA depletion)
  • Ultra low input RNA
  • small RNA
  • RNA Exome


  • Reduced Representation Bisulfite Sequencing (RRBS)
  • ChIP-sequencing


  • 16s and other loci

Service Rates

View internal and external service rates

New Policies and Procedures

Effective January 01, 2021

External Users: Contact Melissa Stephens. We are accepting a limited number of requests until further notice.

Internal Users: Follow all University Re-opening procedures. Access Galvin Life Science Center via the main entrance during regular business days and hours. 

  1. iLab transition complete as of January 06, 2021. Go to InsideND and search for iLab Core Facility Management. GBCF iLab video tutorials and user guide available on Resource tab.

  2. Communicate with facility staff by email, phone, or Zoom appointment. In-person meetings are restricted until further notice.

  3. Samples delivered contactless. Access to facility space is restricted to facility staff.  On designated days and reserved times, there will be a cooler outside the facility door to receive samples. Do not leave samples if the cooler is absent; we are not responsible for damage or loss.

Submitting Your Order

Consultation is required for this service. Before initiating your experiment, contact Melissa Stephens to discuss project specifics, receive a quote, and coordinate scheduling. Once your request is confirmed and scheduled, follow the instructions for library preparation below. 

Library Preparation Submissions

Confirmed and Scheduled Requests

Core Facility will perform its own evaluation of the samples to confirm (1) concentration, (2) purity, and (3) average fragment length to assess quality using a combination of Qubit Assay and fragment analysis (Bioanalyzer or agarose gel).

  1. Project Information: Download and complete all fields to request guide discussion and development of your request.

  2. Library Prep and Sequencing Form: Download and complete all fields to submit your samples for evaluation.

    • Sample requirements are specific to the project and will be outlined during the discussion and development phase prior to confirming and scheduling.

  3. iLab Core Facility Management: Reserve a sample drop-off day/time. iLab sends us a notification about scheduled reservations. Do not send us a separate email. 

    • Go to InsideND, search for iLab Core Facility Management, and sign in using University of Notre Dame credentials.  

    • Schedule a reservation 24 hours in advance by selecting a day and time from the Sample Processing Calendar and attach your completed Excel Submission Form. Within the service request, including the email of other lab members who should be consulted or notified with results. Within the reservation, include the email of other lab members who should be consulted or notified with results.

      • REQUIRED: If you can not make your reservation, cancel it as soon as possible and select a new time slot.

      • Recommendation: Coordinate within your lab to reduce multiple deliveries to a single drop-off reservation whenever possible.

  4. Deposit the sample inside the cooler outside of the facility door on your reserved day and time.

  5. Turn around time is provided during the discussion and development phase. After samples are approved for library contraction and scheduled, the typical turn-around time or 4 to 6 weeks but may be longer for custom projects with modified workflows.